👉 NEET Biology Examination
👉 NEET Biology Tips
👉 NEET Biology Study Material
👉 UGC Net Study Material
Instruction
- Total 15 question
- Total Marks 60
- Each Question 4 marks
- Negative marking -1 for each wrong Question
NEET CONCEPT TEST -10 | BIOLOGY | STD 12 | CHAPTER -11
1. Which of the following methods is used to check the progression of a restriction enzyme digestion ?
( a ) Chromatography ( b ) Polymerase chain reaction
( c ) Agarose gel electrophoresis ( d ) None of these
2. Which of the following is the correct sequence of making a competent bacterial cell ?
( a ) Treatment with divalent cation → incubation in ice with recombinant DNA → heat shock ( 42ºC ) → placing it on ice
( b ) Heat shock ( 42ºC ) → incubation in ice with recombinant DNA → treatment with divalent cation placing it on ice
( c ) Treatment with divalent cation → placing it on ice heat shock ( 42ºC ) → incubation in ice with recombinant DNA
( d ) Incubation in ice with recombinant DNA → heat shock ( 42 ° C ) → treatment with divalent cation → placing it on ice
3. Which one of the given options correctly identifies certain component ( s ) of vector PBR322 ?
( a ) ori - original restriction enzyme
( b ) rop - reduced osmotic pressure
( c ) Hind III , ECOR I - selectable markers
( d ) amp resistance , tet resistance - antibiotic resistance genes
4. If a person obtains transformants , by inserting a recombinant DNA within the coding sequence of B - galactosidase , he will separate out recombinants from non - recombinants by observing that
( a ) non - recombinant colonies do not produce any colour , whereas recombinants form blue coloured colonies
( b ) recombinant colonies do not produce any colour , whereas non - recombinants form blue coloured colonies
( c ) recombinant colonies do not produce any colour whereas non - recombinants form pink coloured colonies
( d ) no colonies are formed due to insertional inactivation .
5. Electroporation facilitates introduction of foreign DNA into the target organism by
( a ) changing the porosity of the cell wall
( b ) changing the electric potential of the cell wall
( c ) lysis of the cell wall
( d ) active transport across the cell wall .
6. Palaeontologists unearthed a human skull during excavation . A small fragment of the scalp tissue was still attached to it . Only little DNA could be extracted from it . If the genes of the ancient man need to be analysed , the best way of getting sufficient amount of DNA from this extract is
( a ) subjecting the DNA to gel electrophoresis
( b ) treating the DNA with restriction endonuclease
( c ) hybridising the DNA with a DNA probe
( d ) subjecting the DNA to polymerase chain reaction .
7. The figure given below shows three steps ( A , B , C ) of Polymerase Chain Reaction ( PCR ) . Select the option giving correct identification together with what represents ?
( a ) Chromatography ( b ) Polymerase chain reaction
( c ) Agarose gel electrophoresis ( d ) None of these
2. Which of the following is the correct sequence of making a competent bacterial cell ?
( a ) Treatment with divalent cation → incubation in ice with recombinant DNA → heat shock ( 42ºC ) → placing it on ice
( b ) Heat shock ( 42ºC ) → incubation in ice with recombinant DNA → treatment with divalent cation placing it on ice
( c ) Treatment with divalent cation → placing it on ice heat shock ( 42ºC ) → incubation in ice with recombinant DNA
( d ) Incubation in ice with recombinant DNA → heat shock ( 42 ° C ) → treatment with divalent cation → placing it on ice
3. Which one of the given options correctly identifies certain component ( s ) of vector PBR322 ?
( a ) ori - original restriction enzyme
( b ) rop - reduced osmotic pressure
( c ) Hind III , ECOR I - selectable markers
( d ) amp resistance , tet resistance - antibiotic resistance genes
4. If a person obtains transformants , by inserting a recombinant DNA within the coding sequence of B - galactosidase , he will separate out recombinants from non - recombinants by observing that
( a ) non - recombinant colonies do not produce any colour , whereas recombinants form blue coloured colonies
( b ) recombinant colonies do not produce any colour , whereas non - recombinants form blue coloured colonies
( c ) recombinant colonies do not produce any colour whereas non - recombinants form pink coloured colonies
( d ) no colonies are formed due to insertional inactivation .
5. Electroporation facilitates introduction of foreign DNA into the target organism by
( a ) changing the porosity of the cell wall
( b ) changing the electric potential of the cell wall
( c ) lysis of the cell wall
( d ) active transport across the cell wall .
6. Palaeontologists unearthed a human skull during excavation . A small fragment of the scalp tissue was still attached to it . Only little DNA could be extracted from it . If the genes of the ancient man need to be analysed , the best way of getting sufficient amount of DNA from this extract is
( a ) subjecting the DNA to gel electrophoresis
( b ) treating the DNA with restriction endonuclease
( c ) hybridising the DNA with a DNA probe
( d ) subjecting the DNA to polymerase chain reaction .
7. The figure given below shows three steps ( A , B , C ) of Polymerase Chain Reaction ( PCR ) . Select the option giving correct identification together with what represents ?
( a ) A - Denaturation at a temperature of about 94-96 ° C separating the two DNA strands
( b ) B - Denaturation at a temperature of about 50 °
( C ) B - Extension in the presence of DNA polymerase
( d ) C- Annealing with two sets of primers
8. Restriction enzymes present in several microorganism , cut foreign DNA at specific sites and destroy them . These enzymes do not destroy the cellular DNA because
( a ) the cellular DNA does not have the specific sites
( b ) the susceptible specific sites are masked by proteins
( c ) the restriction enzyme susceptible sites are modified by addition of methyl group
( d ) the restriction enzymes and DNA occupy different compartments .
9. It was found that Hind II always cuts DNA molecules at a particular point by recognising a specific sequence of six base pairs . This specific base sequence is known as the
( a ) recognition sequence ( b ) restriction endonuclease
( c ) palindromic nucleotide sequence ( d ) exonucleases .
10. Which of the following statements incorrectly explains steps of the normal process during the cloning of recombinant DNA in bacteria ?
( a ) Restriction enzymes are used to cut DNA fragments from cellular and plasmid DNA .
( b ) A copy of the required gene is inserted into a bacterial plasmid by using DNA ligase .
( c ) The Southern blot technique is used to separate the recombinant DNA plasmids .
( d ) The bacteria is transformed by the recombinant DNA plasmids .
11. In the sparged stirred - tank bioreactor
( a ) the surface area for oxygen transfer is increased
( b ) the surface area for oxygen transfer is decreased
( c ) non - sterile air bubbles are sparged
( d ) sterile air bubbles are clustered .
12. Select the incorrect match .
( a ) PCR - A rapid way to amplify DNA in the laboratory
( b ) Vector - A molecule used to carry foreign genes into bacteria
( c ) Gel electrophoresis – Separate DNA fragments based on their size
( d ) Recombinant DNA - Agene sequence from one origin -
13. Read the following statements and select the correct option , Statement A : Recombinant DNA technology has become successful because of the presence of restriction endonucleases in eukaryotic cells . Statement B : Restriction endonucleases cut the DNA molecule to form blunt ends .
( a ) Statement A is correct but statement B is incorrect .
( b ) Statement A is incorrect but statement B is correct .
( c ) Both statements A and B are correct .
( d ) Both statements A and B are incorrect .
14 . Having become an expert on gel electrophoresis , you are asked to examine a gel for a colleague . Where would you find the smallest segments of DNA ?
( a ) Near the positive electrode , farthest away from the wells
( b ) Near the negative electrode , close to the wells
( c ) Near the top , near the negative pole
( d ) Near the middle , they tend to slow down after the first few minutes
15. Significance of heat shock method in bacterial transformation is to facilitate ( a ) binding of DNA to the cell wall
( b ) uptake of DNA through membrane transport proteins
( c ) uptake of DNA through transient pores in the bacterial cell wall
( d ) expression of antibiotic resistance gene .
( a ) the cellular DNA does not have the specific sites
( b ) the susceptible specific sites are masked by proteins
( c ) the restriction enzyme susceptible sites are modified by addition of methyl group
( d ) the restriction enzymes and DNA occupy different compartments .
9. It was found that Hind II always cuts DNA molecules at a particular point by recognising a specific sequence of six base pairs . This specific base sequence is known as the
( a ) recognition sequence ( b ) restriction endonuclease
( c ) palindromic nucleotide sequence ( d ) exonucleases .
10. Which of the following statements incorrectly explains steps of the normal process during the cloning of recombinant DNA in bacteria ?
( a ) Restriction enzymes are used to cut DNA fragments from cellular and plasmid DNA .
( b ) A copy of the required gene is inserted into a bacterial plasmid by using DNA ligase .
( c ) The Southern blot technique is used to separate the recombinant DNA plasmids .
( d ) The bacteria is transformed by the recombinant DNA plasmids .
11. In the sparged stirred - tank bioreactor
( a ) the surface area for oxygen transfer is increased
( b ) the surface area for oxygen transfer is decreased
( c ) non - sterile air bubbles are sparged
( d ) sterile air bubbles are clustered .
12. Select the incorrect match .
( a ) PCR - A rapid way to amplify DNA in the laboratory
( b ) Vector - A molecule used to carry foreign genes into bacteria
( c ) Gel electrophoresis – Separate DNA fragments based on their size
( d ) Recombinant DNA - Agene sequence from one origin -
13. Read the following statements and select the correct option , Statement A : Recombinant DNA technology has become successful because of the presence of restriction endonucleases in eukaryotic cells . Statement B : Restriction endonucleases cut the DNA molecule to form blunt ends .
( a ) Statement A is correct but statement B is incorrect .
( b ) Statement A is incorrect but statement B is correct .
( c ) Both statements A and B are correct .
( d ) Both statements A and B are incorrect .
14 . Having become an expert on gel electrophoresis , you are asked to examine a gel for a colleague . Where would you find the smallest segments of DNA ?
( a ) Near the positive electrode , farthest away from the wells
( b ) Near the negative electrode , close to the wells
( c ) Near the top , near the negative pole
( d ) Near the middle , they tend to slow down after the first few minutes
15. Significance of heat shock method in bacterial transformation is to facilitate ( a ) binding of DNA to the cell wall
( b ) uptake of DNA through membrane transport proteins
( c ) uptake of DNA through transient pores in the bacterial cell wall
( d ) expression of antibiotic resistance gene .
===========================================
If anyone want to join my WhatsApp mail me on my mail ID
indiabiologymanishmevada@gmail.com
ANSWER KEY
1. ( c ) 2. ( a ) 3. ( d ) 4 . ( b ) 5. ( a ) 6. ( d ) 7. ( a ) 8. ( c ) 8. ( c ) 9. ( a ) 10. ( c ) 11. ( a ) 12. ( d ) 12. ( d ) 13. ( d ) 14. ( a ) 15. ( c )
========================================
Mail- indiabiologymanishmevada@gmail.com
Please do not enter any spam link or word in the comment box